TABLE 1

Specific activities of rLipY and rLipY(ΔPE) on various lipid substratesa

SubstrateSpecific activityb (U/mg)
rLipYrLipY(ΔPE)rLipY(ΔPE)/rLipY
Monobutyrin (b) (MC4)61.0 ± 2.092.0 ± 5.01.5
Monolein (a) (MC18)6.6 ± 0.517.0 ± 1.02.5
Diolein (a) (DC18)3.5 ± 0.25.6 ± 0.41.6
Tributyrin (b) (TC4)129.0 ± 6.0267.0 ± 24.02.0
Trioctanoin (a) (TC8)20.0 ± 2.038.0 ± 1.01.9
Pomegranate oil TAG4.0 ± 0.24.8 ± 0.31.2
  • a All substrates were assayed at concentrations above their solubility limits. Experiments were performed at 37°C in 15 ml of 2.5 mM Tris-HCl buffer (pH 7.5) containing 300 mM NaCl and 1 (a) or 3 (b) mM NaTDC.

  • b One unit corresponds to 1 μmol of fatty acid released per min. Values are means from 3 independent experiments ± SD.