TABLE 2

Affinity of OhrR variants for ohrO and ohrROa

ProteinohrOohrRO
Kd (nM)nHKd (nM)nH
OhrR2.8 ± 0.80.7 ± 0.2128 ± 251.0 ± 0.04
OhrR-C16A38 ± 41.0 ± 0.1215 ± 191.0 ± 0.2
OhrR-C121A27 ± 100.9 ± 0.3122 ± 141.0 ± 0.1
OhrR-C16AC121A89 ± 100.9 ± 0.1134 ± 181.0 ± 0.1
  • a Data represent the average ± SD from at least three experiments.