Table 1.

Bacterial strains and plasmids used in this study

Strain or plasmidRelevant genotypeDescription, source and/or reference
E. coli strains
 DH5αMCRF mcrA Δ(mrr-hsdRMS-mcrBC)GIBCO BRL
 SM10 λpir thi thr leu tonA lacY supE recA::RP4-2-Tc::Mu (Knr) λpir 48
 BL21(DE3)F ompT hsdS 59
 SAB11 proA leu lacY1 Ent Enterobactin-deficient strain (5)
S. typhimurium strains
 JF2043 fur-1 zbf-5123::Tn10 iroA1::MudJ 30
 SL1344Wild type 40
 SB136 invA 29
 SB801 ΔsitBCD::aphT This study
 SB804 sitB::lacZ SL1344 derivative with pSB1000 integrated in the chromosome
 SB930 sitB::lacZ SL1344 derivative with pSB1005 integrated in the chromosome
 SB833 fur-1 P22HTint[JF2043]→SL1344
 SB835 fur-1 sitB::lacZ SB833 derivative with pSB1005 integrated in the chromosome
 SB836 fur-1 iroA::MudJP22HTint[JF2043]→SB833
Plasmids
 pSB377R6K-derived replicon (41)
 pGP704 48
 pSB857 sitABCD 36
 pSB992 ΔsitBCD::aphT pSB377 backbone
 pSB998 sitABCD pSC101-derived replicon
 pSB1000 sitB::lacZ pSB377 backbone
 pSB1005 sitB::lacZ pGP704 backbone