Table 1.

Bacterial strains and plasmids used in this study

Strain or plasmidDescriptionaSource or referenceb
Strains
S. pyogenes NZ131D. R. Martin, Porirua, New Zealand
E. coli
 DB11Erythromycin sensitive 13
 JM109JM107 recAJ
 DH10B recA1 BRL
Plasmids
 pCRIIApr; cloning vectorInvitrogen
 pUC18::ermAM Emr 44
 pOU1181,500-bp I-PCR product cloned into pCRII; independently isolated colonyThis study
 pOU4241,500-bp I-PCR product cloned into pCRII; independently isolated colonyThis study
 pVA891-2Emr H. Malke, Jena, Germany31
 pOU500 EcoRI fragment from pOU118 consisting of nucleotides 25–599 of the rgg gene cloned into pVA891-2; Emr This study
 pCRII::rgg 1.185-kb PCR product (Rgg-F, Rgg-R) cloned into pCRIIThis study
 pAM401Shuttle vector; Cmr B. Jett, University of Oklahoma HSC, (53)
 pAM401::rgg EcoRV-BamHI 1.18-kb fragment of pCRII::rgg cloned into pAM401This study
  • a Apr, Emr, and Cmr, resistance to ampicillin, erythromycin, and chloramphenicol, respectively.

  • b BRL, Gibco-Bethesda Research Laboratories Inc., Gaithersburg, Md.; HSC, Health Sciences Center.