Table 1.

Bacterial strains and plasmid used in this study

Strain or plasmidRelevant genotype or phenotypeaSource or reference
Strains
V. cholerae
  C6709El Tor, Inaba, wild type; Smr 3, 38
  Peru2C6709 ΔattRS1; Smr 3, 38
  ETR3Peru2 ΔlacZ lacZ::lppPlacPO→ompA2::SREHP-12::ctxB; Smr 33
  JRB14Peru2 ΔirgA irgA::irgP htpGP→stxB ΔlacZ lacZ::htpGP→stxB eaeA; Smr 5
E. coli JM83 ara Δlac-proAB rpsL φ80lacZΔM15; K-12 derivative 13
Plasmid pCS95pUC18-based plasmid containing in-frame LTA and LTB genes with replacement of the AGA sequence encoding arginine-192 of LTA with the glycine-encoding sequence GGA under the control of the lac promoter; identi-cal to previously described pBD95, except for containing the Shine-Dalgarno sequence of human ltxA; Apr 13; this study
  • a Smr, streptomycin resistance; Apr, ampicillin resistance.