Table 1.

Bacterial strains and plasmids used or constructed in this study

Strain or plasmidRelevant characteristicsSource or reference
Strains
 SL1344 S. typhimurium his mutant, mouse virulent strain 28
 BRD509SL1344 aroA aroDmutant 51
 BRD915SL1344 ΔhtrA 15
 GVB311SL1344rpoE::Kmr This study
 SM10λpir E. coli thi-1 thr1 leuB6 tonA21 lacY1 supE44 recA::RP4-2-Tc::MuKmRλpir 30
Plasmids
 pCR IIPCR cloning vectorInvitrogen
 pRDH10λpir-based suicide vector: Tetr Cmr SacBR. Haigh (Leicester University, Leicester, United Kingdom)
 pUC4KKmrcassette 43
 pSH101 rpoE in pCR IIThis study
 pSH102pCR IIrpoE::Kmr This study
 pWSK29Apr, low copy number vector 52a
 pSH103pRDH10rpoE::Kmr This study
 pSH117 rpoE in pWSK29This study