Table 1.

Bacterial strains and plasmids used or constructed in this study

Strain or plasmidRelevant characteristicsSource or reference
Strains
 SL1344S. typhimurium his mutant, mouse virulent strain28
 BRD509SL1344 aroA aroDmutant51
 BRD915SL1344 ΔhtrA15
 GVB311SL1344rpoE::KmrThis study
 SM10λpirE. coli thi-1 thr1 leuB6 tonA21 lacY1 supE44 recA::RP4-2-Tc::MuKmRλpir30
Plasmids
 pCR IIPCR cloning vectorInvitrogen
 pRDH10λpir-based suicide vector: Tetr Cmr SacBR. Haigh (Leicester University, Leicester, United Kingdom)
 pUC4KKmrcassette43
 pSH101rpoE in pCR IIThis study
 pSH102pCR IIrpoE::KmrThis study
 pWSK29Apr, low copy number vector52a
 pSH103pRDH10rpoE::KmrThis study
 pSH117rpoE in pWSK29This study