Table 1.

Bacterial strains and plasmids

Strain or plasmidKnown type III effector protein(s) expressed or relevant characteristic(s)Reference(s) or source
P. aeruginosa strains
 PA103ExoT, ExoUB. H. Iglewski
 PA103ΔexoU ExoT 4
 PA103exoT::TcExoU 3
 PA103ΔexoUexoT::TcNoneThis study
 PA103toxA::ΩExoT, ExoUB. H. Iglewski
 PA103exsA::ΩNone 7
Plasmids
 pMOBexoT::TcAllelic replacement vector encoding tetracycline-interruptedexoT 3, 18
 pUCP18pUC-derived cloning vector able to replicate in P. aeruginosa 17
 pUCPexoS Encodes the wild-type ADP-ribosyltransferase ExoS (100% activity) 12
 pUCPexoSE381AEncodes the noncatalytic mutant ExoSE381A (0.02% activity)This study and 13
 pUCPexoT Encodes the ADP-ribosyltransferase ExoT (0.2% activity) 21
 pUCPexoY Encodes the adenylate cyclase ExoY 23
 pUCPexoYK81MEncodes the noncatalytic mutant ExoYK81M 23