Table 2.

Presence of variant FimP subunit proteins in A. naeslundii and A. viscosus strains of different animal host origins

StrainaBinding specificitybHybridization withfimP DNA probescFimP proteindOrigine
APRPsStatherinType 1 full-length probeType 1:1 central probeType 1:2 central probeType 1:1Type 1:2
A. naeslundiiT14V+++ (72)+ (6)540YesNoHuman periodontal pocket (7)
A. naeslundiiLY7++ (56)+ (1)430YesNoHuman dental plaque (13)
A. naeslundiiP-1-K++ (26)+ (1)440YesNoHuman dental plaque (18)
A. naeslundiiB-1-K+++ (62)+ (3)540YesNoHuman buccal mucosa (18)
A. viscosus ATCC 19246++ (35)+++ (72)305NoYesHuman actinomycosis (51)
A. viscosus35452++ (44)+++ (73)405NoYesRat mouth (CCUG)
A. viscosusR28++ (56)+++ (81)405NoYesRat mouth (57)
A. viscosus35451++ (38)+++ (70)404NoYesHamster mouth (CCUG)
A. viscosus14476++ (37)+++ (80)404NoYesHamster mouth (CCUG)
  • a The isolates were identified by multivariate statistical analyses of phenotypic characteristics, serological reactions, and protein banding patterns of cell extracts analyzed by SDS-PAGE (18).

  • b Binding to APRPs or statherin was scored as strong (+++), moderate (++), or weak (+) based on adhesion of radiolabeled bacteria to protein-coated hydroxyapatite beads. The figures within parentheses give the proportion (percentage of added cells) of bacteria attaching to protein-coated hydroxyapatite beads.

  • c The fimP type 1:1 full-length DNA probe was generated from the entire fimP type 1 fimbrial gene of A. naeslundii T14V, and the fimP central type 1:1 (APRP-binding specificity) and type 1:2 (statherin-binding specificity) DNA probes were generated from the central segments of thefimP type 1 fimbrial genes of A. naeslundii T14V and A. viscosus ATCC 19246, respectively (see reference 20and Materials and Methods). The DNA probes were labeled with digoxigenin and used in slot blot hybridization to probe chromosomal DNA from Actinomyces spp. under high-stringency conditions. The degree of hybridization was scored from 0 to 6 by comparison with a scale based on densitometric measures: 0, 0.01; 1, 0.01 to <0.04; 2, 0.04 to <0.10; 3, 0.10 to <0.16; 4, 0.16 to <0.22; 5, 0.22 to <0.27; and 6, ≥0.27.

  • d Presence of the different FimP subunit proteins based on binding specificity and fimP DNA probe hybridization pattern.

  • e Host and tissue origins of the strains, with references (parentheses).