Table 1.

Sequence identity among fimP subunit genes ofA. naeslundii and A. viscosus with statherin- or APRP-binding specificities

StrainaBinding specificitybDNA identitycSite of isolationd
APRPsStatherin
A. naeslundii
 T14V+++ (72)+ (6)ReferenceHuman periodontal pocket (7)
 LY7++ (56)+ (1)98.1Human dental plaque (13)
 P-1-K++ (26)+ (1)98.6Human dental plaque (18)
 B-1-K+++ (62)+ (3)98.6Human buccal mucosa (18)
A. viscosus ATCC 19246++ (35)+++ (72)83.7Human cervicofacial actinomycosis (51)
  • a The strains were identified by multivariate statistical analyses of phenotypic characteristics, serological reactions, and protein banding patterns of cell extracts analyzed by SDS-PAGE (18).

  • b Binding to APRPs or statherin was scored as strong (+++), moderate (++), or weak (+) based on adhesion of radiolabeled bacteria to protein-coated hydroxyapatite beads. The figures within parentheses give the proportion (percentage of added cells) of bacteria attaching to protein-coated hydroxyapatite beads.

  • c Nucleotide sequence identity (%) of thefimP genes (GenBank accession numbers are given in Materials and Methods) compared to the fimP gene of T14V reference strain (60). Ten regions of comparably high identity (83 to 97%; nucleotide sequences 1 to 210, 226 to 303, 361 to 501, 517 to 576, 595 to 741, 823 to 870, 949 to 1200, 1225 to 1266, 1297 to 1431, and 1456 to 1599) are interspersed with 10 regions of lower identity (38 to 78%; nucleotide sequences 211 to 225, 304 to 360, 502 to 516, 577 to 594, 742 to 822, 871 to 948, 1201 to 1224, 1267 to 1296, 1432 to 1455, and 1600 to 1617). The numbering starts from the bp 1 to 3 ATG start codon after alignment to the fimP gene of strain T14V.

  • d Site of isolation, with a literature reference (in parentheses). The P-1-K and B-1-K strains were from different sites in the same individual but belonged to different ribotypes (19).