Table 1.

Analysis of genes disrupted in urease-negative mutants, as determined by plasmid rescue and subsequent sequencing

MutantSite of insertion (bp)aH. pylori gene no.aSp act of urease (U/mg of protein)bHomologue of interrupted ORF
176,61200720.042 ± 0.001Urease B gene
275,25800710.074 ± 0.003Urease accessory gene I
3256,17102470.050 ± 0.002ATP-dependent RNA helicase gene
  • a According to the sequence of Tomb et al. (37).

  • b Mean from three independent experiments ± standard error of the mean. The urease activity of the parental strain was 34.91 ± 0.043 U/mg of protein.