Table 4.

Flow cytometer analysis of spleen cells from nonimmunized and GK-1-immunized micea

Incubation with:Mean % positive cellsb
ControlsImmunized mice
CD3+CD4+CD8+CD3+CD4+CD8+
Medium38.8 ± 2.726.4 ± 0.8 (5.7)10.9 ± 0.8 (5.1)399 ± 4.028.3 ± 1.2 (5.8)12.2 ± 1.2 (4.5)
GK-138.5 ± 2.226.6 ± 2.08 (4.9)11.7 ± 1.0 (4.3)58.7 ± 4.9b 32.6 ± 2.6b (91.1)19.3 ± 2.3b (93.9)
Cysticercal antigens35.2 ± 2.124.3 ± 0.9 (4.7)10.5 ± 0.6 (3.9)54.7 ± 6.5b 32.7 ± 2.9b (86.6)20.4 ± 3.7b (89.5)
  • a Flow cytometer analysis was performed on spleen cells of nonimmunized and GK-1-immunized mice after 3 days of culture without or with GK-1 or T. crassicepscysticercal antigens. Each value represents the percentage of positive cells from four individual mice and is representative of two experiments performed in duplicate. The percentage of CD4+or CD8+ cells that also expressed CD25+ is given in parentheses.

  • b Significant increase in the percentage of CD8+, CD4+, and CD3+ cells in specifically stimulated immune mice compared with nonimmunized mice (P < 0.05).