Table 1.

Plasmids used in this study

PlasmidCharacteristicsaReference or source
pUC18Apr; ColE1 ori; blue/white; MCS 60
pUC19Apr; ColE1 ori; blue/white; MCS 60
pBK-CMVNeor; SV40, f1 and ColE1 ori; blue/white; MCSStratagene
pHP042Neor; 3.5-kb genomic fragment of H. pylori 17874 in pBK-CMVThis work
pRY109Apr; Cmr; cat gene inBamHI site of pUC18 61
pSP101Apr; 6.3-kbHindIII/BglII genomic fragment of H. pylori 17874 in HindIII/BamHI-cut pUC18This work
pSP102Apr; 7-kbHindIII/BglII genomic fragment of H. pylori 17874 in HindIII/BamHI-cut pUC18This work
pSP105Apr; compositefliQ PCR fragment in SmaI-cut pUC19This work
pSP106Apr; composite fliI PCR fragment in SmaI/HincII-cut pUC19This work
pSP107Apr; Cmr; cat gene from pRY109 in primer introduced BglII site of pSP105This work
pSP110Apr; Cmr;cat gene from pRY109 in primer introduced BamHI site of pSP106This work
pSP116Apr; compositevirB11 PCR fragment in SmaI-cut pUC19This work
pSP118Apr; Cmr; catgene from pRY109 in primer introduced BamHI site of pSP116This work
  • a ColE1, colicin E1 origin of replication; MCS, multiple cloning site; SV40, simian virus 40.