Table 2.

Oligonucleotides used for cloning and sequencing

Gene or transposonPrimerStrandSequenceUse(s)
TnphoA Tnp55′-TCGCTAAGAGAATCACGCAGAG-3′Sequencing
Tn5 Tnp3+5′-GCACGATGAAGAGCAGAAG-3′Sequencing
ibeB IB-5HB+5′-AGGATCCGAGCCTATGTCTCCTTG-3′Cloning, sequencing
ibeB IB7-5a5′-GTAAAGCGCATGGTCATC-3′Cloning, sequencing
ibeB IB7-5b+5′-TTTAATGCCCAGGCTAACG-3′Cloning, sequencing
ibeB IB7-3a+5′-GCCAGCAGCGATCTGTCGTC-3′Cloning, sequencing
ibeB IB7-315′-CGGCGATATCCAGATTG-3′Sequencing
ibeB IB7-325′-TTAGCCGCCATTAACGCGTG-3′Sequencing
ibeB IB7-335′-TTACCCAGCGTTTCTTCG-3′Sequencing
ibeB IB7-3c+5′-ATCAGCAACTGGCGTATG-3′Sequencing
ibeB IB7-345′-TTAACCAGGCCGTTCTGG-3′Sequencing