Table 1.

Oligonucleotide primer sets detecting ACT1 andSAP1 to SAP7 gene mRNAs and 5.8S rRNA

Gene (reference)Accession no.PrimerSequenceRT-PCR product size (bp)Primer positions
SAP1(25) X56867 Upstream5′-TCAATCAATTTACTCTTCCATTTCTAACA-3′96–124
Downstream5′-CCAGTAGCATTAACAGGAGTTTTAATGACA-3′161237–256
SAP2(55) M83663 Upstream5′-AACAACAACCCACTAGACATCACC-3′94–117
Downstream5′-TGACCATTAGTAACTGGGAATGCTTTAGGA-3′178242–271
SAP3(53) L22358 Upstream5′-CCTTCTCTAAAATTATGGATTGGAAC-3′151–176
Downstream5′-TTGATTTCACCTTGGGGACCAGTAACATTT-3′231352–381
SAP4(32) L22388 Upstream5′-CATTCATTCCTTTAATACCGACTATC-3′69–94
Downstream5′-GGTAACAAACCCTGTAGATCTTTTAAC-3′156198–224
SAP5(34)30191UpstreamSame asSAP4 149–174, 174–199, 199–224
DownstreamSame asSAP4 156, 181, 206328–354
SAP6(34)30192UpstreamSame asSAP4 105–130, 130–155, 155–180
DownstreamSame asSAP4 156, 181, 206284–310
SAP7(34)30193Upstream5′-GAAATGCAAAGAGTATTAGAGTTATTAC-3′280–307
Downstream5′-GAATGATTTGGTTTACATCATCTTCAACTG-3′196446–475
ACT1 (29)XI6377Upstream A5′-GGCTGGTAGAGACTTGACCAACCATTTG-3′3042224–2251
Upstream B5′-GATTTTGTCTGAACGTGGTAACAG-3′2712257–2280
Downstream5′-GGAGTTGAAAGTGGTTTGGTCAATAC-3′2502–2527
5.8S rRNA (30)L07796Upstream5′-TCCGTAGGTGAACCTGCGG-3′196–214
Downstream5′-TCCTCCGCTTATTGATATGC-3′534710–729