Table 1.

Immunofluorescent binding of P66- and OspA-specific monoclonal antibodies to B. burgdorferi cells with different Osp phenotypes

IsolateOsp phenotypeaBinding of antibodyb to:
Fixed cellsUnfixed cells
OspAOspBOspCOspDH1337H914H1337H914H5332
B31+++
HB19+++
B314++
HB19R1+++
B313
 Untreated++
 Trypsin treatedc  NDd ND+NDND
 Proteinase K treatedc ND
  • a +, expression of an Osp protein as determined by sodium dodecyl sulfate-PAGE and Western blot analysis.

  • b +, binding in IFA.

  • c Trypsin and proteinase K were used at a concentration of 200 μg/ml of cell suspension.

  • d ND, not done.