TABLE 1.

Bacterial strains and plasmids used in this study

Strain or plasmidRelevant feature(s)Source or reference
Strains
    DH5αLaboratory E. coli strain
    RDEC-1O15:H−, Nalr7
    RDEC-H19ARDEC-1 transduced with phage H19A, Nalr Tetr46
    SM10SM10 λpir, recipient for suicide vector pCVD44213
    TSA01SM10 containing pM381This study
    RDEC-1Δeae860-939RDEC-1Δeae860-939, NalrThis study
Plasmids
    pALT417-3pALTER-1::RDEC-1eae, Ampr
    p368Derived from pALT417-3 with 1-bp deletion in eae, AmprThis study
    pCVD442Suicide vector, Ampr13
    pM381pCVD442::Δeae, Kanr AmprThis study