TABLE 1.

Bacterial strains and plasmids used in this study

Strain or plasmidDescriptionReference or source
Strains
    Serovar Typhimurium
        CS09314028sATCC
        CS40114028s phoN2 zxx::6251 Tn10d-Cm Strra30
        JAF43CS401 ΔsseJ Camr Strr15
        JAF57CS401 ΔsifA Camr Strr15
        JAF65CS401 ΔsifA ΔsseJ Camr Strr15
        MBO86CS093 with pKD46, AmprThis work
        MBO87CS093 ΔsseJ CamrThis work
        MBO93MBO87 with pWSK129, Camr KanrThis work
        MBO95MBO87 with pKF35, Camr AmprThis work
        MBO96MBO87 with pKF36, Camr AmprThis work
        MBO97MBO87 with pKF37, Camr AmprThis work
        MBO98CS093 with pWSK129, KanrThis work
        MBO99CS093 with pWSK29, AmprThis work
        MBO100MBO87 with pWSK29, Camr AmprThis work
        MBO106MBO87 with pMBO76, Camr AmprThis work
        MBO107MBO87 with pJAF111, Camr AmprThis work
        MBO121JAF65 with pJAF111, Camr Strr AmprThis work
        MBO125JAF65 with pMBO76, Camr Strr AmprThis work
    E. coli
        CS371BL21(DE3) with pLysS gold, CamrNovagen
        JAF018CS371 with pJAF08, Camr AmprThis work
        KF71CS371 with pKF33, Camr AmprThis work
        KF72CS371 with pKF27, Camr AmprThis work
        KF73CS371 with pKF28, Camr AmprThis work
        KF74CS371 with pKF29, Camr AmprThis work
Plasmids
    pKD46λ Red recombinase vector, Ampr13
    pWSK29Low-copy-number expression vector, Ampr40
    pWSK129Low-copy-number expression vector, Kanr40
    pET-15bSix-His tag cloning vector, AmprNovagen
    pJAF08Six-His-SseJ in pET-15b, AmprThis work
    pKF27Six-His-SseJ-S153A in pET-15b, AmprThis work
    pKF28Six-His-SseJ-H384N in pET-15b, AmprThis work
    pKF29Six-His-SseJ-S151A in pET-15b, AmprThis work
    pKF33Six-His-SseJ-D247N in pET-15b, AmprThis work
    pJAF111SseJ-HA in pWSK29, Ampr15
    pKF35SseJ-S151A-HA in pWSK29, AmprThis work
    pKF36SseJ-D247N-HA in pWSK29, AmprThis work
    pKF37SseJ-H384N-HA in pWSK29, AmprThis work
    pMBO76SseJ-S151A-SseJ-D247N-SseJ-H384N-HA in pWSK29, AmprThis work
  • a Data unclear.