TABLE 1.

Strains, plasmids, and bacteriophages used in this study

Strain plasmid, or virusDescriptionSource or reference
P. aeruginosa strains
    1244Wild-type strain, group I pili34
    1244G7PilO-negative mutantThis study
    1244wbpLO-antigen-negative mutant15
    1244N3rpoN33
    9D2Wild-type strain, group I pili9
    577BWild-type strain, group I pili9
    653AWild-type strain, group I piliC. C. Brinton, University of Pittsburgh
    134VAWild-type strain, group I piliC. C. Brinton, University of Pittsburgh
    PA103Wild-type strain, group II pili9
    PAKWild-type strain, group II pili9
    T2AWild-type strain, group II pili9
    Isolate collectionClinical isolatesS. D. Kominos, Mercy Hospital, Pittsburgh
Plasmids
    pC110Apr Gmr, pUC7 derivative50
    pEX18TcTcr oriT SacB, allelic replacement vector39
    pGIL100Apr, pNOT322 containing pilAO operonThis study
    pGIL101Apr, pGIL100, EcoRI/XbaI fragment removedThis study
    pGIL200Apr, Gmr, 1.3-kb replacement of pilO DNAThis study
    pGIL300Tcr, sacB, PstI/HindIII fragment from pPAC124GMThis study
    pMBT100Apr, 1.2-kb insert containing 1244 pilO under tac promoter in pMMB66EHThis study
    pMMB66EHApr, broad-host-range vector17
    pNOT332Apr Tcr, cloning vector38
    pPAC24Apr, 0.8-kb insert containing strain 1244 pilA under tac promoter in pMMB66EH7
    pPAC46Apr, 2.3-kb insert containing strain 1244 pilAO under tac promoter in pMMB66EH7
    pPAC124Apr, 2.3-kb insert containing strain 1244 pilAO under tac promoter in pUC187
    pPAC202Tcr, subclone containing strain 1244 pilAO operon10
    pUC46GMApr Gmr, pPAC124 with 1.3-kb replacement of pilO DNAThis study
    pUCP19exoUspcUApr, pUC19-based plasmid carrying exoU and spcU1
    pT7-7Apr, cloning vector containing T7 RNAP promoter49
Viruses
    PE69, PO4, M6,P. aeruginosa pilus-specific RNA bacteriophages6
    F116P. aeruginosa pilus-specific RNA bacteriophages22