TABLE 2.

T-cell response the day before genital challengea

AntigenAdjuvantT-cell proliferation response (103 cpm) to:In vitro cytokine production
IFN-γ (pg/ml)IL-4 (pg/ml)TNF-α (pg/ml)
EBbConAcMediumEBbConAcEBbConAcEBbConAc
MOMPCpG + Montanide10.3 ± 2.3d66.5 ± 1.60.2 ± 0.053,973 ± 1d2,510 ± 128<0.858 ± 3459 ± 41d318 ± 0
OvalbuminCpG + Montanide0.9 ± 0.181.0 ± 9.10.09 ± 0.01561 ± 143,341 ± 34<0.843 ± 2302 ± 5288 ± 8
MOMPCpG + alum6.1 ± 2.2d73.7 ± 9.40.3 ± 0.32,097 ± 26d3,266 ± 02 ± 0.752 ± 1372 ± 34d310 ± 24
OvalbuminCpG + alum1.7 ± 0.540.3 ± 6.21.1 ± 0.51,361 ± 152,575 ± 334<0.866 ± 3262 ± 9254 ± 15
C. trachomatis17.2 ± 2.1d57.2 ± 21.30.08 ± 0.033,081 ± 137d2,309 ± 2095 ± 0.275 ± 3455 ± 14d398 ± 10
MEM-02.2 ± 0.748.4 ± 6.60.2 ± 0.08683 ± 102,957 ± 81<0.850 ± 0.1164 ± 6236 ± 55
  • a Results are the means for triplicate cultures (±1 standard deviation). Data correspond to one of the experiments representative of duplicate separate experiments.

  • b UV-inactivated C. trachomatis MoPn EB were added at a 10:1 ratio to the antigen-presenting cells.

  • c Concanavalin A (ConA) was added at a concentration of 5 μg/ml.

  • d P < 0.05 by the Student t test, compared to the ovalbumin- or MEM-0-immunized group.