TABLE 1

Strains and primers used in this study

Strain, plasmid, or primerDescription/nucleotide sequence (5′ to 3′)Reference or source
Strains
    ICC169Wild-type C. rodentium O152 serotype36
    ICC180Bioluminescent C. rodentium O152 serotype36
    ICC1333C. rodentium espO deletion mutant, KanRThis study
Plasmids
    pKD46Coding for the lambda Red recombinase37
    pKD4Coding for the kanamycin resistance cassette37
    pICC929pET28a expressing C. rodentium EspA with a C-terminal 6-histidine tagThis study
Primers
    EspO(W77A)-FwGCCTCAGAAGCTCAGGCGATGTGCAAAATTATAG
    EspO(W77A)-RvCTATAATTTTGCACATCGCCTGAGCTTCTGAGGC
    k1-EspO-del-FwGCCGGATAACGGTGTACGCTGTATGTAATACAACCAGGAGGAAATCGATGCTGTGTAGGCTGGAGCTGCTTCG
    k2-EspO-del-RvGCAGACATCGGCACAATCCCTGTGGCGGTATCTGCCCGAAAAATCGTCAGGACATATGAATATCCTCCTTAGTTCC
    Ext-EspO-check-FwTGTATTGCATTAACAGGATCAC
    Ext-EspO-check-RvAGCTTTATGTATGTGTGCCTG
    EspA-CR-NcoI-FwCATGCCATGGATACATCAACTATGACATCAGTTGC
    EspA-CR-EcoRI-RvCGGAATTCGGTTTGCCAATGGGTATTGCTGAAACAG