TABLE 3

Strains and plasmids used in this study

Strain and genotype or plasmidDescriptionSource or reference
Strains
    Y. pseudotuberculosis
        wtIP32953 serotype IE. Carniela
        wtLwt 16S rRNA::luxABCDE EryrThis study
        tatC::cmlIP32953 with tatC insertion mutation, Cmlr22
        ΔtatCIP32953 with tatC in-frame deletion23
        ΔtatCLIP32953 ΔtatC 16SrRNA::luxABCDE, EryrThis study
        sufI::cmlIP32953 with sufI insertion mutation, Cmlr23
        ΔsufIIP32953 with sufI in-frame deletionThis study
        ΔsufILIP32953 ΔsufI 16SrRNA::luxABCDE, EryrThis study
        ΔcynTIP32953 with cynT in-frame deletionThis study
        ΔycbKIP32953 with ycbK in-frame deletionThis study
        ΔYPTB3260IP32953 with YPTB3260 in-frame deletionThis study
        ΔYPTB1325IP32953 with YPTB1325 in-frame deletionThis study
        fhuD::cmlIP32953 with fhuD insertion mutation, Cmlr23
        cueO::cmlIP32953 with cueO insertion mutation, Cmlr23
        amiC::cmlIP32953 with amiC insertion mutation, Cmlr23
        ybtP::cmlIP32953 with ybtP insertion mutation, Cmlr23
        yedY::cmlIP32953 with yedY insertion mutation, CmlrThis study
        efeB::cmlIP32953 with efeB insertion mutation, CmlrThis study
        pepP::cmlIP32953 with pepP insertion mutation, CmlrThis study
        nicO::cmlIP32953 with nicO insertion mutation, CmlrThis study
        dmsA::cmlIP32953 with dmsA insertion mutation, CmlrThis study
        napA::cmlIP32953 with napA insertion mutation, CmlrThis study
        dmsA2::cmlIP32953 with dmsA2 insertion mutation, CmlrThis study
        tauA::cmlIP32953 with tauA insertion mutation, CmlrThis study
        fdoG::cmlIP32953 with fdoG insertion mutation, CmlrThis study
        YPTB3074::cmlIP32953 with YPTB3074 insertion mutation, CmlrThis study
        urtA::cmlIP32953 with urtA insertion mutation, CmlrThis study
        YPTB2692::cmlIP32953 with YPTB2692 insertion mutation, CmlrThis study
        YPTB0523::cmlIP32953 with YPTB0523 insertion mutation, CmlrThis study
        ΔcynT::cmlIP32953 with cynT in-frame deletion and YPTB0523 insertion mutation, CmlrThis study
        ΔycbK::cmlIP32953 with ycbK in-frame deletion and YPTB0523 insertion mutation, CmlrThis study
        ΔYPTB3260::cmlIP32953 with YPTB3260 in-frame deletion and YPTB0523 insertion mutation, CmlrThis study
        ΔYPTB1325::cmlIP32953 with YPTB1325 in-frame deletion and YPTB0523 insertion mutation, CmlrThis study
    E. coli
        S17-1 λpirRP4-2 Tc::Mu-Km::Tn7pir)c61
        TOP10F mcrAΔ(mrr-hsdRMS-mcrBC) ϕ80dlacZΔM15 ΔlacX74 recA1 araD139Δ(ara leu)7697 galU galK rpsL (Strr) endA1 nupGInvitrogen
Plasmids
        pNQ705Suicide integration vector, Cmlr62
        pUA1pNQ705 with bases 80–391 of fhuD, Cmlr23
        pUA2pNQ705 with bases 80–391 of cueO, Cmlr23
        pUA3pNQ705 with bases 125–437 of sufI, Cmlr23
        pUA4pNQ705 with bases 100–413 of amiC, Cmlr23
        pUA5pNQ705 with bases 146–315 of ybtP, Cmlr23
        pUA6pNQ705 with bases 118–441 of yedY, CmlrThis study
        pUA7pNQ705 with bases 25–373 of efeB, CmlrThis study
        pUA8pNQ705 with bases 20–354 of pepP, CmlrThis study
        pUA9pNQ705 with bases 28–339 of nicO, CmlrThis study
        pUA10pNQ705 with bases 133–443 of dmsA, CmlrThis study
        pUA11pNQ705 with bases 135–445 of napA, CmlrThis study
        pUA12pNQ705 with bases 124–434 of dmsA2, CmlrThis study
        pUA13pNQ705 with bases 27–333 of tauA, CmlrThis study
        pUA14pNQ705 with bases 147–457 of fdoG, CmlrThis study
        pUA15pNQ705 with bases 78–456 of YPTB3074, CmlrThis study
        pUA16pNQ705 with bases 36–483 of urtA, CmlrThis study
        pUA17pNQ705 with bases 19–272 of YPTB2692, CmlrThis study
        pUA066pNQ705 with bases 71–310 of YPTB0523, Cmlr57
        pDM4Suicide vector, Cmlr63
        pDM4cynTΔcynT in pDM4, CmlrThis study
        pDM4tatCΔtatC in pDM4, Cmlr23
        pDM4sufIΔsufI in pDM4, CmlrThis study
        pDM4ycbKΔycbK in pDM4, CmlrThis study
        pDM4YBTP3260ΔYBTP3260 in pDM4, CmlrThis study
        pDM4YBTP1325ΔYBTP1325 in pDM4, CmlrThis study
        pBAD24Expression vector, araB, Ampr64
        pBAD24-sufIss-mCherrypBAD24 with sufIss::mCherry, AmprThis study
        pCR2.1-TOPOCloning vector, AmprInvitrogen
        pCR2.1-TOPO-ybtP-FLAGPCR2.1-TOPO with ybtP::FLAGThis study
        pMMB207Expression vector, tac, Cmlr65
        pMMB207-yptP-FLAGpMMB207 with ybtP::FLAG, CmlrThis study
  • a Institute Pasteur, Paris, France.