TABLE 1

Putative Tat substrates predicted by PRED-TAT analysis

Physiological roleCellular localizationY. pseudotuberculosis
GenBank accession no.Protein
Respiration
    Nitrate reductionCytoplasmicYP_071269NapFa
    Nitrate reductionPeriplasmicYP_071267NapA
    DMSOb reductionInner membraneYP_069346DmsA
    DMSO reductionInner membraneYP_071198DmsA2
    Formate oxidationPeriplasmicYP_072403FdoG
    Putative iron-sulfur protein?YP_071202YPTB2692
Cell cycle
    Cell divisionPeriplasmicYP_071879SufI
    Cell wall amidasePeriplasmicYP_071528AmiC
Iron acquisition
    Transport proteinInner membraneYP_070126YbtP
    Iron-hydroxamate transporterPeriplasmicYP_069281FhuD
Transport
    Nickel transportInner membraneYP_069066YPTB0523
    Nickel/cobalt transportInner membraneYP_071442NicO
    Urea transportPeriplasmicYP_069763UrtA
    Sugar transportPeriplasmicYP_069857YPTB1325
    Taurine transporterPeriplasmicYP_072203TauA
    Cysteine transporterPeriplasmicYP_070244FliYc
Others
    Putative sulfatasePeriplasmicYP_071579YPTB3074
    TetR family regulatory protein?YP_068741YPTB0195a
    Multidrug translocaseInner membraneYP_072411MdfAc
    Carbonic anhydrase?YP_071573CynT
    Proline-specific aminopeptidase?YP_071686PepP
    Sulfite oxidoreductase/protein repairPeriplasmicYP_072050YedY/MsrP
    Copper homeostasisPeriplasmicYP_069263CueO
    Deferrochelatase/peroxidasePeriplasmicYP_070253EfeB
Unknown
    Hypothetical proteinExported/outer membraneYP_071754YPTB3261c
    Hypothetical proteinExported/outer membraneYP_071753YPTB3260
    Hypothetical proteinExported/outer membraneYP_069963YcbK
  • a Removed from the list.

  • b DMSO, dimethyl sulfoxide.

  • c Deletion was unsuccessful.