Table 1.

Bacterial strains, plasmids, and oligonucleotides used in this study

Strain, plasmid, or oligonucleotidesGenotype, markers, sequenceSource or reference
Strains
E. coliDH5αF-, thi-1f80dlacZΔM15 Δ(lacZYA-argF)U169 deoR recA1 endA1 hsdR17(rk mk +) supE44 gyrA96 relA Bethesda Research Laboratories—Life Technologies
E. coliNM522F′, lacI q Δ(lacZ)M15 proA+ B+ supE thiΔ(lac-proAB) Δ(hsdMS-mcrB)5 (rk mk +)mcrBC New England Biolabs
N. gonorrhoeae1291Our collection
P. aeruginosaC 14
P. aeruginosaPAO-1 35
Plasmids
 pGEM3zf+Ampicillin resistantPromega Corp.
 pPSC-1Ampicillin resistantThis study
 pPSC-2Ampicillin resistantThis study
 pPSC-3Ampicillin resistantThis study
 pPSC-4Ampicillin resistantThis study
Oligonucleotides
 T7 primerPromega Corp.
 SP6 primerPromega Corp.
 PSC-3 OLIGO 15′-ATG GCT CTT GAT AGT TCG GG-3′
 PSC-3 OLIGO 25′-CCC GAA CTA TCA AGA GCC AT-3′
 A12 SP6-EXT5′-AGT TTC AGC GCG TTG AGT-3′
 A12 SP6-OLIGO 15′-CGG GAT GAT CTT TAT CGG AA-3′