Table 1.

In vitro ADP-ribosyltransferase activities of wild-type and mutant strains of P. aeruginosa

StrainActivities (ADPRT/OD540)a in:
Bacteriological mediumbTissue culture mediumc
SupernatantdLysateeSupernatantLysate
388138.6 ± 28.13.5 ± 1.256.0 ± 17.770.7 ± 39.9
388pscC::Tn1 00.1 ± 0.13.7 ± 2.188.2 ± 16.2
388pscC::Tn5Tc00.1 ± 0.13.2 ± 2.973.7 ± 13.5
388pscN::Tn5Tc003.9 ± 1.9105.6 ± 7.4
388popD::TcΩ58.0 ± 7.91.6 ± 0.332.0 ± 11.635.6 ± 12.9
  • a Values provided are the average and standard deviation for experiments performed in triplicate and are reported as 10−18 moles of ADPRT per OD540.

  • b Bacterial cultures were grown at 32°C in a deferrated dialysate of trypticase soy broth as detailed in Materials and Methods.

  • c Bacterial cultures were grown in the presence of serum at 37°C.

  • d Activity is measured from the soluble portion of a bacterial culture subjected to centrifugation.

  • e Activity measured from the soluble portion of a bacterial extract. Bacteria were lysed by sonication.