Table 4.

Effect of pilD mutations on TCP production

Recipient strain and growth conditionAggTransduction frequency witha:
CTXφ704ACTXφ-Km
O395 (TCP-inducing conditions)+9.1 × 10−3 ± 2.0 × 10−3 9.1 × 10−3 ± 2.7 × 10−3
O395 (non-TCP-inducing conditions)<2.8 × 10−7 ± 1.1 × 10−7
BGD4 (ΔtcpA)<6.0 × 10−8 ± 0.1 × 10−8 <4.2 × 10−8 ± 0.1 × 10−8
KP8.97 (tcpB::TnphoA)<9.7 × 10−8 ± 0.1 × 10−8
KFV5R (pilD::kanπ, reverted)4.1 × 10−5 ± 0.3 × 10−5
KFV5R (pKJF308Cm) + 0.05% arabinose+4.5 × 10−2 ± 1.7 × 10−2
KFV5R (pKJF308Cm) + no arabinose1.0 × 10−3 ± 0.4 × 10−3
KFV32 (ΔpilD)3.9 × 10−5 ± 0.4 × 10−5
KFV32 (pKJF308) + 0.05% arabinose+1.4 × 10−2 ± 0.2 × 10−2
KFV32 (pKJF308) + no arabinose2.5 × 10−4 ± 0.8 × 10−4
  • a Transduction frequency is the CFU of transductants divided by the CFU of recovered recipients. Numbers given are averages ± standard deviations from duplicates in a single experiment.