Table 1.

Oligonucleotide primers and probes used in this study

DesignationSequencePurpose
US-475′-GAATGTAACAAAATTATATATTTAAATCTTTGAAAAAT-3′Probe for ospE/F loci
fla5′5′-GATTATATCAATCATAATACATCAGC-3′Specific probe
ospC 5′-CTTATTAAGGTTTTTTTGGACTTTCTGC-3′Specific probe
ospF 5′-TACTATAGAAAATAATGGAAAAG-3′Specific probe
bbk2.11 5′-ATCTAAATTAGATGAAAAAG-3′Specific probe; PCR
2.10-5′5′-AAAAAAGAAGAGTTGGTTGGTGGTTT-3′ bbk2.10-specific primer
2.10-3′5′-AAACACCTTGAGCCACTTGCTC-3′ bbk2.10-specific primer
p21 5′-GCAAAGTAATAATGAGTTAAAAGTTAAGCAAAG-3′Specific probe; PCR
ospE 5′-GGAGACTCTGATACATTCGCAG-3′Specific probe; PCR
elpA1 5′-CAATAGTAGGCGAAATAGCAAG-3′Specific probe; PCR
elpA2 5′-GGAAAGAAATAAAAATGTTGTAGGG-3′Specific probe; PCR
ospA 5′-TATGTTCTTGAAGGAACTCTAACTGCTGAAAAAACAACATT-3′Specific probe
Cons-DS5′-CCCTTTACTATTTTTGTAGTTGCTTTTGC-3′Downstream PCR primer
2.9-5′consL5′-AAAGGAGTAACAATGAAAATYATCAAC-3′a Long-distance PCR
ospE-R+C5′-CCTGCGAATGTATCAGAGTCTCC-3′Long-distance PCR
p21-R+C5′-CCCGGAATCCTTAATTTTATTTCC-3′Long-distance PCR
ospF-LD5′-CATCTATAACTTTGTTTGCCAATTCATTGC-3′Long-distance PCR
  • a Y represents a C or T nucleotide.