Table 1.

PCR amplification primers

Amplification (reference)Primer pairs (5′-3′)DirectionBase position (amplicon length [bp])
ragA.1 TGT CTG GTG CGA TAG CGA ATA G Forward3198–4904 (1,600)
TAC ATA GGT GAG TTT GAG ATT C Reverse
ragB.1 AAT ACT GAA AAT CCA CGA Forward5275–6686 (1,500)
TAG GGG CTG CGA CAA AAA Reverse
ragA.2 CAA CCA CCA AGC ACT CGT Forward4033–4542 (509)
CGA CTT GAC CAG GAA CAT AC Reverse
ragB.2 GAC TCT TGG CTC GTT TAC TG Forward6001–6436 (436)
ACG CAA ACG ACT ACC CTC A Reverse
P. gingivalis 16S RNA (4) AGG CAG CTT GCC ATA CTG CG Forward729–1132 (404)
ACT GTT AGC AAC TAC CGA TGT Reverse
P. gingivalis rpgA catalytic domain (3) AGT GAG CGA AAC TTC GGA GC Forward1199–2902 (1,709)
CCT GTC CAA TAT GGG TCA CTA TGG Reverse