Table 1.

Plasmids and bacterial isolates used in this study

Plasmid or bacteriumDescriptionReference(s) or source
 pPCP19.6-kb pesticin (Pst) plasmid encoding Pla 26, 47
 pCD170.6-kb low calcium response (Lcr) plasmid 26, 47
 pMT1∼100-kb murine toxin/fraction 1 (Tox) plasmid 26, 47
 pBR322Cloning vector 41
 pEK4Vector portion of pBR322 ligated to the cloned 1.45-kbHindIII-SmaI fragment of pPCP from Y. pestis 358 encoding Pla under control of its own promoter 6
 p2PCP::Tn 1pPCP::Tn 1 (insert in silent region)This study
 pVK1pPCP::Tn 1 (insert in silent region) 33
 pVK2pPCP::Tn 1 (insert inpst) 33
E. coli
 LE392F hsdR5149(rm) supE44 supF58 lacY1 galK2 galT22 metB1 trpR55 λ 41
 K802F hsdR2(r Km+ K) supE44 lacY1 galK2 galT22 metB1λ American Type Culture Collection
Y. pseudotuberculosis
 PB1/+pYV; serotype IB 12
 PB1/0from PB1/+ 12
 PB1/+ (B44) trp bio gua, pYV; from PB1/+This study
 PB1/0 (B45) trp bio gua; from B44This study
 PB1/+ (B53) trp bio gua, pYV, pPCP; from B44This study
 PB1/0 (B59) trp bio gua, pPCP; from B53This study
 85pYV; serotype IIICulture collection of Institute “Microbe”
 85/1pYV, pPCP (pVK1)This study
 85/2pYV, pPCP (pVK2)This study
 861pYV; serotype IIH. Molleret
 861/1pYV, pPCP (pVK1)This study
 861/2pYV, pPCP (pVK2)This study
Y. pestis
 KIM (D27)Δpgm,a pPCP1, pCD1, pMT1 7
 KIM (D28)Δpgm, pPCP1, pMT1From D27
 KIM (D45)Δpgm, pCD1, pMT157
 KIM (D47)Δpgm, pMT1From D45
 KIM (D39)Δpgm, pPCP1, pCD1, pMT1, Met+ b 56
 KIM (D49)Δpgm, pCD1, pMT1, Met+ From D39
 KIM (D40)Δpgm, pPCP1, pMT1, Met+ From D39
 KIM (D50)Δpgm, pMT1, Met+ From D49
  • a Δpgm, expression of avirulent nonpigmented phenotype (29) due to ∼100-kb spontaneous chromosomal deletion (47).

  • b Met+, methionine meiotroph (21).