Table 2.

Isotypes and subtypes of antibodies to B. pertussis whole cells and LPS

Serum sample no.aIgMIgG3IgG1IgG2IgG4IgA
CellsbLPScCellsLPSCellsLPSCellsLPSCellsLPSCellsLPS
 1++++++++
 2++++++++
 3++++++++/−+++
 4++++++
13+++++++/−+++
 5+/−+/−+++++
 6++
 7++++++
 8++++++
 9++++++
10++
11++++++
12++/−++++
  • a Samples 1 through 5 and sample 13 had bactericidal activity against the wild-type strain and the complement-sensitive mutant, and samples 6 through 12 had activity against only the complement-sensitive mutant.

  • b Whole cells of wild-type BP338 and LPS mutant MLT7 were boiled in SDS loading buffer and transferred to polyvinylidene difluoride membranes for Western blotting as described in the legend for Fig. 8.

  • c The wild-type strain BP338 and the LPS mutant strain MLT7 were treated with proteinase K to remove protein antigens as described in the legend for Fig. 8, and antibodies to the terminal trisaccharide of LPS were identified as reactivity at the appropriate molecular mass, which is present in BP338 but lacking in MLT7.