Table 1.

Transendothelial migration of neutrophils and monocytes in response to infection with C. trachomatis and C. pneumoniae

TreatmentaLevel of transendothelial migration (cells/HPF) ofb:
NeutrophilsMonocytes
Mock5.9 ± 0.62.9 ± 0.4
C. trachomatis L2
 Live9.4 ± 1.77.0 ± 1.6*
 Heat inactivated8.0 ± 1.64.9 ± 0.9
 UV inactivated12.0 ± 1.34.8 ± 0.6
C. pneumoniae TW-183
 Live22.8 ± 3.2**12.9 ± 1.2***
 Heat inactivated10.5 ± 1.14.7 ± 0.6
 UV inactivated16.0 ± 1.1**6.2 ± 1.1
  • a HUVEC monolayers cultured in transwells were inoculated with C. trachomatis L2 or C. pneumoniae TW-183 at a multiplicity of infection of 1:1. Equivalent inoculum concentrations of each strain were inactivated by heat or UV light before infection (see Materials and Methods). Mock-infected cells were treated with a suspension of lysed HEp-2 cells.

  • b Levels of neutrophil and monocyte transendothelial migration were determined following 24 h of incubation as described in Materials and Methods. Data represent means ± standard errors of the means of three separate experiments. In each experiment, triplicate wells were assayed separately for each condition for neutrophil and monocyte migration . *, P < 0.05; **, P < 0.01; ***, P < 0.001.