Table 2.

Adherence of labeled B. pertussis Tohama I and W28, with or without FITC or SYTO 9, to NCI-H292 and HEp-2 epithelial cells

Labeling of bacteriaMean no. of bacteria/100 epithelial cells ± SD (n ≥ 3)
NCI-H292HEp-2
Tohama IW28Tohama IW28
FITC82 ± 1452 ± 1576 ± 253 ± 14
SYTO 971 ± 1152 ± 1162 ± 948 ± 22
Nonea 94 ± 1353 ± 1970 ± 652 ± 20
  • a After adherence of unlabeled bacteria to epithelial cells, the bacteria were stained with SYTO 9 (see Materials and Methods).