Table 2.

Secretion of IL-8 and MCP-1 by HUVEC infected withC. trachomatis and C. pneumoniae

TreatmentaConcn (ng/ml )b
IL-8MCP-1
Mock0.32 ± 0.110.3 ± 0.08
C. trachomatis
 Serovar L20.16 ± 0.050.3 ± 0.17
 Serovar A0.13 ± 0.020.28 ± 0.05
 Serovar E0.08 ± 0.040.28 ± 0.09
C. pneumoniae TW-18320.4 ± 0.9*5.0 ± 1.3*
  • a HUVEC monolayers were inoculated withC. trachomatis serovars L2, A, and E or C. pneumoniae TW-183 at a multiplicity of infection of 1:1. Mock-infected cells were treated with a suspension of lysed HEp-2 cells.

  • b Levels of IL-8 and MCP-1 from culture supernatants were measured following 24 h of incubation by ELISA. Data indicate the means ± standard errors of the means of three separate experiments. In each experiment, duplicate wells were assayed separately for each condition for IL-8 and MCP-1 production . *, P < 0.001.