Table 5.

Infant mouse colonization by pilD mutants of classical and El Tor V. cholerae strains

Competing strainsaInoculum ratiobIn vitro ratiocIn vivo colonizationd
No. of miceAvg CFU recoveredRatio (mutant/ΔlacZ strain)
MutantΔlacZstrain
KFV5R and Lac10.987.9 × 10−5 403.4 × 106 ± 4.2 × 106 <2.9 × 10−7
3.81.3 × 10−3 502.3 × 106 ± 3.8 × 106 <4.3 × 10−7
41.30.056405.4 × 105 ± 4.9 × 105 <1.9 × 10−6
O395N1 alone51.6 × 107 ± 1.8 × 107
KFV5R alone50
KFV18R and KFV100.391.5 × 10−4 401.4 × 108 ± 0.9 × 108 <7.3 × 10−9
2.54.0 × 10−4 401.2 × 108 ± 1.1 × 108 <8.6 × 10−9
20.90.02851.6 × 104 ± 1.8 × 104 2.6 × 107 ± 2.6 × 107 6.2 × 10−4
P4 alone42.5 × 104 ± 1.2 × 104
KFV18R alone9≤3.5 × 103
  • a For a description of strains, see Table1.

  • b For classical strains, inocula were adjusted to 1 × 106 to 2 × 106/ml (total dosage) at the ratios indicated, while El Tor strains were adjusted to 3 × 105 to 8 × 105/ml (total dosage) at the ratios indicated.

  • c Competitive growth in LB broth overnight at 37°C.

  • d CFU recovered represent the total CFU in 5 ml of prepared intestinal extract ± standard deviations.