TABLE 7

Oligonucleotides used in this study

NameUse5′–3′ sequence (size [bp])
P2Signature tag region PCRATCCTACAACCTCAAGCT (18)
P4Signature tag region PCRATCCCATTCTAACCAAGC (18)
STM13-KE-complement-1-RevWild-type Ap3AS PCRCCACAGAGAACTTGAAG (17)
STM13-KF-CWild-type Ap3AS PCRTATAAACCTGGTACGG (16)
IGstmGenmeF3DNA sequencing and ST mutant PCRGGACTGTTATATGGCCTTTTTGGATC (26)
STM03-B-RevST mutant PCRACAGCTTGACGTTTTCCA (18)
STM18-RevST mutant PCRAGCAAAATTTCCACCCAAGA (20)
STM20-RevST mutant PCRGAGACATCAATCGCGGTTTT (20)
JZ-STM22ST mutant PCRCTCAATTGAAGAATTATATGATG (23)
STM26-RevST mutant PCRCATCCCACCTGAAAACTCGT (20)