Table 1.

Integrated density values (IDV) for immunoreactive proteins identified by 2D-NEPHGE immunoblotting

ProteinMolecular massaIDVb
pH 6.0pH 7.0pH 8.0
OspA31.425,05627,66622,968
OspC25.415,18025,9901,150
I-197.1451656820
I-260.21,2601,530990
I-450.21,7001,870595
I-744.95,1667,0560
I-844.74,8006,3000
I-944.07,6966,6600
I-1044.08101,0800
I-1143.206801,115
I-1243.29031,8064,386
I-1343.82,8563,7800
I-1443.82,7363,3120
I-1540.82856842,166
I-1736.252811440
I-1835.12,8475,1830
I-1933.66,5868,2776,586
I-2032.97803600
I-2128.01,8922,1503,440
I-2226.32,2681,998378
I-2325.44,8064,6280
I-2425.49,16710,6803,837
I-2523.02,9643,8760
I-2621.75,2894,1280
I-2721.47,0302,5652,565
I-2821.410,7007,8112,461
I-2921.427,41625,0248,464
I-3018.43,9502,0542,528
I-3216.35,4025,5501,702
I-3317.03,2255,8054,902
  • a Molecular mass in kilodaltons compared to molecular mass standards (Fig. 3).

  • b IDV = Σ(each pixel value − background).