Table 3.

H. ducreyi hemolysin is cytotoxic to fibroblasts and human epithelial cells

StrainNormalized LDH releasea from:
HFFHFEHEp-2 cells
350000.08 ± 0.000.20 ± 0.010.10 ± 0.01
35000ΔAPC0.02 ± 0.010.18 ± 0.030.05 ± 0.01
35000ΔAPC(pLSSK)0.02 ± 0.000.17 ± 0.010.05 ± 0.01
35000ΔAPC(pLSBA+2)19.25 ± 3.4928.37 ± 8.692.74 ± 0.20
35000ΔAPC(pLS384-2)59.78 ± 2.8588.70 ± 13.463.98 ± 0.25
35000ΔAPC(pLSB+)0.01 ± 0.000.14 ± 0.040.08 ± 0.04
35000ΔAPC(pLS376−)0.04 ± 0.00NDb ND
  • a Values represent normalized LDH release (see Materials and Methods) and are the average of quadruplicate samples (HFFs were assayed in triplicate) ± standard error. The results of a typical experiment are shown; the experiment was repeated at least three times with similar results.

  • b N.D., not determined.