TABLE 2

Classification of transposon insertion sites in all ST mutants in this study

Gene category and ST mutantaTransposon insertion siteFunction
Cell envelope, membranes and lipoproteins
    11-1, -2, and -3MGA_0964VlhA 4.02 (hemagglutinin)
    17-1 and -2MGA_0379VlhA 3.02 (hemagglutinin)
    13-1, -2, and -3No match in strain RlowMatch to vlhA gene in draft Ap3AS sequence
Cellular processes, 01-1, -2, and -3MGA_1142OsmC-like stress-induced protein
Translation, protein synthesis, 19-1, -2, and -3MGA_0216Elongation factor P (EF-P)
Transport and binding proteins, ABC transport
    20-1, -2, and -3MGA_0220ATP-binding protein OppD1
    24-1, -2, and -3MGA_0221Permease protein OppC1
    26-1, -2, and -3MGA_0220ATP-binding protein OppD1
    36-1, -2, and -3MGA_0223Permease protein OppB1a
Intergenic regions
    10-1, -2, and -3MGA_0537–MGA_0539HsdM endonuclease, hsd1 locus
    16-1, -2, and -3MGA_0395–MGA_0398vlhA 3.09, mal P
    23-1, -2, and -3MGA_0379–MGA_0380vlhA 4.04, vlhA 4.05
    27-1, -2, and -3MGA_0226–MGA_0230Conserved hypothetical protein, oppF (OppF)
    31-1, -2, and -3MGA_0071–MGA_0073vlhA 1.05, putative transposase
    38-1, -2, and -3MGA_0518–MGA_0519Unique hypothetical protein, conserved hypothetical protein
Others
    02-2MGA_0549Unique hypothetical protein
    02-3MGA_0145Putative transposase
    03-1, -2, and -3MGA_1102Hypothetical membrane protein, zinc peptidase motif
    04-3MGA_0073Putative transposase
    07-1, -2, and -3MGA_0554Hypothetical lipoprotein
    09-1 and -2MGA_0662Unique hypothetical membrane protein
    12-1, -2, and -316S rRNA16S rRNA
    14-1, -2, and -3MGA_0549Hypothetical membrane protein unique to M. gallisepticum and M. synoviae
    18-1, -2, and -3MGA_0588Hypothetical membrane protein, multiple paralogs
    21-1, -2, and -3MGA_0554Hypothetical lipoprotein
    22-1, -2, and -3MGA_1079Hypothetical lipoprotein
    32-1, -2, and -3MGA_0817bUnique hypothetical protein
    35-1, -2, and -3MGA_0758Putative hemoxygenase
Not determined
    09-3Multiple insertions
    15-1, -2, and -3Multiple insertions
    17-3Multiple insertions
    25-1, -2, and -3Multiple insertions
    34-1, -2, and -3Multiple insertions
  • a The following mutants have been described previously (5): 02-1; 04-1; 06-1, -2-, and -3; 28-1, -2, and -3; 33-1, -2, and -3; and 39-1, -2, and -3.