Table 5.

H. ducreyi hemolysin is cytotoxic to human immune system cell lines

StrainNormalized LDH releasea from:
U937 cellsJurkat cellsDaudi cellsRaji cells
350000.35 ± 0.051.08 ± 0.070.21 ± 0.010.12 ± 0.01
35000ΔAPC0.08 ± 0.020.07 ± 0.000.09 ± 0.020.08 ± 0.01
35000ΔAPC(pLSSK)0.18 ± 0.070.04 ± 0.010.06 ± 0.030.03 ± 0.02
35000ΔAPC(pLSBA+2)5.67 ± 0.3073.63 ± 2.831.91 ± 0.181.16 ± 0.19
35000ΔAPC(pLS384-2)69.62 ± 10.42225.95 ± 17.2230.09 ± 0.124.23 ± 0.23
35000ΔAPC(pLSB+)0.17 ± 0.040.02 ± 0.050.06 ± 0.020.06 ± 0.02
  • a Values represent normalized LDH release and are the average of quadruplicate samples ± standard error. The results of a typical experiment are shown; the experiment was repeated at least three times with similar results.