Table 1.

Plasmids used in this study

PlasmidRelevant descriptionReference
pACYC184Cloning vector, p15A replication origin7
pET109tib locus cloned into pHG16515
pET139tibA gene (in pHG165) under transcriptional control of an endogenous promoter15
pET140tibA gene under transcriptional control of the lac promoter found in pHG16515
pET146tibB,tibC, and tibD ORFs cloned into pACYC18415
pHC79Cosmid cloning vector23
pHG165pBR322 copy number derivative of pUC8; ColE1 replication origin38