Table 1.

Bacterial strains and plasmids used in this study

Bacterial strain or plasmidRelevant characteristicsReference or source
Strains
E. coliHB101Noninvasive strain, negative control18
S. typhimurium SL1344Highly invasive strain, positive control16
H. influenzae RdNonhemolytic, noncytotoxic, negative control, ATCC 33391M. Roberts, University of Washington
H. ducreyi
 35000Wild type, hemolytic41
 35000ΔAPC35000 hhdA::cat, nonhemolytic, CmrThis study
Plasmids
 pPT376pUC19 with hhdA, Apr41
 pBluescript KS(−)Cloning vector, AprStratagene
 pBluescript SK(−)Cloning vector, AprStratagene
 pPT376KShhdAon pBluescript, AprThis study
 pPT376ΔPstpPT376KS with internal hhdA PstI fragment replaced with BamHI linkers, AprThis study
 pUC-ΔEcatSource ofcat cassette, CmrB. Green, Lederle-Praxis, Rochester, N.Y. (13)
 pPT376ΔPstCmpPT376ΔPst with hhdA::cat, AprCmrThis study
 pLS88H. ducreyishuttle vector, Kmr Smr48
 pBFDDeletion derivative of pLS88, SmrThis study
 pCR2.1TA cloning vector, AprKmrInvitrogen, San Diego, Calif.
 pLSSKH. ducreyi shuttle vector, SmrThis study
 pLSKSH. ducreyishuttle vector, SmrThis study
 pBABNhhdBA on pUC1911
 pLSBA+hhdBA on pLSSK, SmrThis study
 pLSBA+2hhdBA on pLSSK in same orientation aslac promoter, SmrThis study
 pLSBAHishhdBA fused to His tag on pLSSK11
 pLSB+pLSSK with hhdB in same orientation aslac promoter, SmrThis study
 pPT384-TZ18pTZ18R with hhdBA in same orientation as lac promoter, Apr41
 pLS384−hhdBA insert of pPT384-TZ18 in pLSKS opposite the lac promoter, SmrThis study
 pLS384-2hhdBA on pLSSK in opposite orientation oflac promoter, SmrThis study
 pLS376−hhdA on pLSSK in opposite orientation oflac promoter, SmrThis study