Table 1.

Bacterial strains and plasmids used in this study

Bacterial strain or plasmidRelevant characteristicsReference or source
Strains
E. coliHB101Noninvasive strain, negative control18
S. typhimurium SL1344Highly invasive strain, positive control16
H. influenzae RdNonhemolytic, noncytotoxic, negative control, ATCC 33391M. Roberts, University of Washington
H. ducreyi
 35000Wild type, hemolytic41
 35000ΔAPC35000 hhdA::cat, nonhemolytic, Cmr This study
Plasmids
 pPT376pUC19 with hhdA, Apr 41
 pBluescript KS(−)Cloning vector, Apr Stratagene
 pBluescript SK(−)Cloning vector, Apr Stratagene
 pPT376KS hhdAon pBluescript, Apr This study
 pPT376ΔPstpPT376KS with internal hhdA PstI fragment replaced with BamHI linkers, Apr This study
 pUC-ΔEcat Source ofcat cassette, Cmr B. Green, Lederle-Praxis, Rochester, N.Y. (13)
 pPT376ΔPstCmpPT376ΔPst with hhdA::cat, AprCmr This study
 pLS88 H. ducreyishuttle vector, Kmr Smr 48
 pBFDDeletion derivative of pLS88, Smr This study
 pCR2.1TA cloning vector, AprKmr Invitrogen, San Diego, Calif.
 pLSSK H. ducreyi shuttle vector, Smr This study
 pLSKS H. ducreyishuttle vector, Smr This study
 pBABN hhdBA on pUC1911
 pLSBA+ hhdBA on pLSSK, Smr This study
 pLSBA+2 hhdBA on pLSSK in same orientation aslac promoter, Smr This study
 pLSBAHis hhdBA fused to His tag on pLSSK11
 pLSB+pLSSK with hhdB in same orientation aslac promoter, Smr This study
 pPT384-TZ18pTZ18R with hhdBA in same orientation as lac promoter, Apr 41
 pLS384− hhdBA insert of pPT384-TZ18 in pLSKS opposite the lac promoter, Smr This study
 pLS384-2 hhdBA on pLSSK in opposite orientation oflac promoter, Smr This study
 pLS376− hhdA on pLSSK in opposite orientation oflac promoter, Smr This study