Table 2.

Primers used in this study

Primer nameSequenceaNucleotide recognition site (accession no.)
A start 5′-gga tcc aaG GAG ATA CAT ATA TGA AAA AAT GGA-3′ 1885–1909 (U32175)
rev A end 5′-gtc gac TCG AAT GGC CAT CTT AGC ATC GAC-3′ 5398–5421 (U32175)
88R 5′-aga tca aga tcT GCA ATG CAA GGT CGC CAA CAC-3′ 2991–3010 (L23118)
88BFD 5′-aga tca aga tct CTG CCG CAC AGC TCC ATA GG-3′ 4711–4730 (L23118)
5′BlueMCS 5′-gat gat cAG CGG ATA ACA ATT TCA CAC AGG-3′ 827–846 (X52324)
3′BlueMCS 5′-gat gat cAC GCT TAC AAT TTC CAT TCG C-3′ 451–470 (X52324)
NcoF 5′-ATG CAA ATG GTA TAA AAG TTA G-3′ 5077–5099 (U32175)
A end Sal 5′-gtc gac CCA CTT ACA TCA ATC-3′ 5576–5591 (U32175)
  • a Primer sequences homologous to the template are shown in capital letters, non-homologous sequences are in lowercase letters, and restriction sites incorporated into primers to facilitate the cloning of PCR products are underlined.