Table 4.

Survival of strain LF82 within HEp-2 and Intestine-407 cells

Gentamicin treatment period (h)Intracellular survival (mean ± SD [n ≥ 3])
HEp-2Intestine-407
CFU × 105/wella% Relative CFUb% CellscCFU × 105/well% Relative CFU% Cells
1690.0 ± 42.4100100203.5 ± 60.1100100
21,240.0 ± 56.6179.8 ± 5.0 NDd 283.5 ± 65.8140.7 ± 9.2ND
41,530.0 ± 183.9223.0 ± 40.4ND198.0 ± 45.398.3 ± 6.8ND
61,720.0 ± 537.4247.4 ± 62.799.8218.5 ± 34.6109.6 ± 15.4157.6
241,685.0 ± 459.6242.6 ± 51.7114.5163.3 ± 34.981.3 ± 6.9159.3
  • a Number of CFU recovered from lysed monolayers.

  • b Percent intracellular bacteria relative to the number after 1 h of gentamicin treatment, defined as 100%.

  • c Percent epithelial cells remaining attached to the plates relative to the number after 1 h of gentamicin treatment, defined as 100%.

  • d ND, not determined.