Table 1.

Frequency of leukocyte accumulation within blood vesselsa

C. perfringens strainTotal no. of blood vesselsNo. (%) of vessels with:
Moderate leukocyte accumulationSevere leukocyte accumulation
Wild-type heat-killed (JIR325)c 2019 (4.5)0
Wild-type (JIR325)b 47846 (9.6)41 (8.6)
Theta-toxin-deficient (JIR4081)c 48527 (5.6)3 (0.6)
Alpha-toxin-deficient (JIR4120)b,c 46672 (15.5)14 (3.0)
  • a Analysis of the frequency of leukocyte accumulation within blood vessels of tissue samples taken 8 to 12 h postinjection. Moderate leukocyte accumulation indicates that the vessel had a single layer of leukocytes lining the luminal surface of the blood vessel (an example of such a vessel is indicated in Fig. 1I). Severe leukocyte accumulation indicates multiple leukocyte layers (an example of such a vessel is indicated in Fig. 1E).

  • b Differs significantly from tissues injected with heat-killed C. perfringens cells (P < 0.0001).

  • c Differs significantly from tissues injected with wild-type C. perfringens cells (P < 0.0001).