TABLE 1

Bacterial strains used in this study

StrainsRelevant characteristic(s)aSource
E. faecalis
    OG1RFReference sequenced strain; Rifr FusrLaboratory stock
    ΔrelEfHigh basal (p)ppGpp; SR negativeb6
    ΔrelQEfDelayed SRb6
    ΔrelEf ΔrelQEf(p)ppGpp0b6
    ΔcodYcodY deletionThis study
    ΔrelEf ΔrelQEf ΔcodY(p)ppGpp0, codY deletionThis study
    ΔcodY + pcodYThis study
    ΔrelEf ΔrelQEf ΔcodY + pcodYThis study
    V583Reference sequenced strain22
    V583 ΔrelEf ΔrelQEf(p)ppGpp022
    CK111OG1Sp upp4::P23repA447
E. coli
    EC1000Host for RepA-dependent plasmids48
  • a Fusr, fusidic acid resistance; Rifr, rifampin resistance.

  • b For details, see Gaca et al. (7).