Table 3.

Internalization of bovine and human S. aureusisolates by MAC-T and adherence to immobilized Fn

StrainaPercentage of inoculum internalized in:Fn adherence indexd
Medium aloneMedium + 100 nM Fnb
8325-42.5 ± 0.10.001 ± 0.0014.3 ± 0.19
DU58830.1 ± 0.070.1 ± 0.050.1 ± 0.01
DU5883(pFnBPA4)11.2 ± 0.7NDc 10.8 ± 0.4
Novel17.8 ± 1.61.0 ± 0.044.0 ± 0.3
30512.9 ± 0.40.3 ± 0.025.3 ± 0.4
Newman1.5 ± 0.10.02 ± 0.0061.3 ± 0.05
MN818.0 ± 0.80.1 ± 0.021.0 ± 0.02
190950.5 ± 0.030.002 ± 0.0010.11 ± 0.01
  • a Strains are described in Table 1.

  • b The purity of commercially obtained bovine Fn was confirmed by analyzing 20 μg of protein per lane of sodium dodecyl sulfate-polyacrylamide gel electrophoresis gels. The preparation resolved into a homogeneous protein band.

  • c ND, not determined.

  • d Calculated as described in Materials and Methods.