Table 2.

Abilities of strain LF82 and of reference strains belonging to the different pathogenic groups of E. coli to invade HEp-2 cells

E. coli strainPathogenic groupInvasion efficiencya (mean ± SD [n ≥ 3])
MOI = 10MOI = 100
CFU × 105/wella% InvasionbCFU × 105/well% Invasion
LF82160.5 ± 143.54.01 ± 3.59423.6 ± 355.01.06 ± 0.89
E12860/0EIEC121.2 ± 198.13.03 ± 4.95489.6 ± 204.41.22 ± 0.51
E2348/69EPEC131.7 ± 168.33.29 ± 4.21111.9 ± 132.50.28 ± 0.33
H10407ETEC72.1 ± 57.01.80 ± 1.42100.8 ± 101.40.25 ± 0.25
C1845DAEC24.6 ± 30.00.61 ± 0.7520.4 ± 12.70.05 ± 0.03
EDL933EHEC7.4 ± 5.40.18 ± 0.1316.5 ± 9.10.04 ± 0.02
17-2EAggEC3.7 ± 3.00.09 ± 0.0811.5 ± 10.00.03 ± 0.03
K-12 C6000.04 ± 0.040.001 ± 0.0011.4 ± 1.30.004 ± 0.003
  • a Number of CFU recovered from lysed monolayers.

  • b Percentage of inoculum surviving gentamicin treatment.