Table 2.

Expression of costimulatory molecules in IFN-γ KO micea

TreatmentΔMCFb
CD28 on CD4+ cellsCD28 on CD8+ cellsB7.2 on T cells
OVA/alum466215
B. abortus20829
B. abortus-OVA20730
  • a IFN-γ KO mice were immunized with the antigens shown, and spleen cells were assessed for expression of CD28 or B7.2 40 h later.

  • b ΔMCF is the MCF for a particular marker after subtraction of the MCF obtained from the background staining with the isotype-matched control antibody. MCFs for the isotype-matched antibody for each treatment were similar. CD4+ and CD8+ T cells were gated by using anti-CD4 or anti-CD8 antibodies; T cells were gated by staining with anti-CD3.