Table 1.

Oligonucleotides used in this study

OligonucleotideaNucleotide sequencebDescription
4505′-CGTTGGATCCAGCTGTCCGCAGGCG-3′ nirBupstream region, 5′ PCR primer
4515′-CTGACTGCAGCCATGGTTGCCTCGATTTC-3′ nirBupstream region, 3′ PCR primer
6635′-CTCTGGATCCTAATGGGTTTTATAGCG-3′ pagCupstream region, 5′ PCR primer
6645′-AAAGAATTCTTTCCATGGCAACTCCTTAAT-3′ pagCupstream region, 3′ PCR primer
MU15′-CCGGGATCCAGCTGTTACAACTCATATTG-3′ katGupstream region, 5′ PCR primer
MU25′-ATCGTCTGCAGTGCCCATGGCTCAGCTCCCTTTTA-3′ katGupstream region, 3′ PCR primer
MU55′-ACGGATCCTACCATGGAAGACGCCAAAAAC-3′Firefly luciferase, 5′ PCR primer
MU65′-CGTCATCGCTGGATCCAGTAAGCTTTTACAATTTGGACTTTCC-3′Firefly luciferase, 3′ PCR primer
MU75′-CGGGATCCCATGGAAAATCTGGATTGTTGGG-3′C fragment, 5′ PCR primer
29475′-AAACTGCAGTTAGTCGTTGGTCCAACCTTC-3′C fragment, 3′ PCR primer
30095′-CACGCTCATCGATAATTTCACC-3′Truncated β-galactosidase, 3′ PCR primer
30135′-CCGCCGCTCGAGGCCCATGGCCATGATTACGGATTCACTG-3′Truncated β-galactosidase, 5′ PCR primer
  • a MU series oligonucleotides were synthesized in the Department of Microbiology and Immunology, The University of Melbourne, Parkville, Victoria Australia; all other oligonucleotides were synthesized and purchased from the Microbial Biotechnology and Diagnostic Unit, Monash University, Clayton, Victoria Australia.

  • b Restriction enzyme sites are underlined.