Table 1.

E. coli strains and plasmids used in this study

Strain or plasmidGenotype or other relevant characteristicsDerivationReference or source
E. coli strains
 CP9O4/K54/H5Clinical blood isolate 36
 CP9.I-2 ure1::TnphoA′1Tn10tet::proΔlacZ, activelacZ fusionKanr exconjugant of BW16948 × CP917 37, this study
 CPI-2 ure1::TnphoA′1 T4 (CP9.I-2) × CP9This study
 CP9.45 iroNE. coli ::TnphoA, active TnphoAfusionKanr exconjugant of CP9/pRT291 × MM294/pPH1J1 36, this study
 CP45 iroNE. coli ::TnphoA T4 (CP9.45) × CP9This study
 CP9.82 iroNE. coli ::TnphoA, active TnphoAfusionKanr exconjugant of CP9/pRT291 × MM294/pPH1J1 36, this study
 CP82 iroNE. coli ::TnphoA T4 (CP9.82) × CP9This study
 CP9.274 artJ::TnphoA, active TnphoA fusionKanr exconjugant of CP9/pRT291 × MM294/pPH1J1 36, this study
 CP274 artJ::TnphoA T4 (CP9.274) × CP9This study
 XL1-Blue recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac (F+ proAB lacI q ZΔM15 Tn10)Stratagene
Plasmids
 pCPI-2.18.8-kbSalI/EcoRI fragment from CPI-2 containing the leftward 5.0 kb of TnphoA (active fusion) and a portion ofure1 cloned into pBSII SK(−)This study
 p45.121-kb SalI/SacI fragment from CP45 containing the leftward 5.0 kb of TnphoA (active fusion) and a portion of iroNE. coli cloned into pBSII SK(−)This study
 p45.322-kbClaI/XbaI fragment from CP45 containing the rightward 6.7 kb of TnphoA (nonfusion) and a portion ofiroNE. coli cloned into pBSII SK(−)This study
 p82.122-kb BamHI/SacI fragment from CP82 containing the leftward 5.0 kb of TnphoA (active fusion) and a portion of iroNE. coli cloned into pBSII SK(−)This study
 p274.17.4-kbBamHI/XbaI fragment from CP274 containing the leftward 5.0 kb of TnphoA (active fusion) and a portion ofartJ cloned into pBSII SK(−)This study