Table 1.

Results of infectivity assays 4 weeks postchallenge

ImmunogenNo. of mouse samples positive for infection/ no. testeda% Protection
E. coli with pBluescript vector only5/50
Recombinant OspC-pBluescript
 Nondenatured1/16b 93.75
 Heat denatured4/520
Recombinant OspC-pSCREEN
 Nondenatured (insoluble inclusions)3/540
 Heat denatured5/50
 Guanidine denatured5/50
 His tag column purified (guanidine-HCl removed)5/50
  • a Two assays, ear biopsy culture and Western blot, were performed, with identical results.

  • b Combined results from two studies.